What is Epigenetic?
Biological mechanisms in which DNA, RNA, and proteins are chemically or structurally modified, without changing their primary sequence (a.k.a mutation).
Epigenetic regulatory mechanisms include DNA methylation and
hydroxymethylation, histone modification, chromatin remodeling, RNA
methylation, and regulation by small and long non-coding RNAs.
Is it normal for our body to have DNA methylation?
Yes, it is normal for our body, in fact recently many diseases due to aberration in epigenetic function.
In complicated terms these epigenetic modifications normally play critical roles in the regulation of
numerous cellular processes, including gene expression, DNA replication,
and recombination
Source: Zymo
Source: uiuc.edu
What kind of disease due to epigenetic aberration?
Well the most obvious is cancer.
What is DNA methylation?
DNA which has methyl group (-CH3) on its nucleotide (especially Cytosine or C in mammals)
Source: uiuc.edu
Or in complicated term DNA methylation: Methylation of cytosine is a covalent modification of DNA, in which hydrogen H5 of cytosine is replaced by a methyl group
What is bisulfite treatment?
Converting C to be U by using sodium metabisulfite
What is bisulfite protocol?
What kind of kit to choose?
Zymo Research has several kit depend on your need, the following is recommended to see.
Source:
Zymo Research
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